Publication Date:
2008-01-19
Description:
Dynein and kinesin motor proteins transport cellular cargoes toward opposite ends of microtubule tracks. In neurons, microtubules are abundantly decorated with microtubule-associated proteins (MAPs) such as tau. Motor proteins thus encounter MAPs frequently along their path. To determine the effects of tau on dynein and kinesin motility, we conducted single-molecule studies of motor proteins moving along tau-decorated microtubules. Dynein tended to reverse direction, whereas kinesin tended to detach at patches of bound tau. Kinesin was inhibited at about a tenth of the tau concentration that inhibited dynein, and the microtubule-binding domain of tau was sufficient to inhibit motor activity. The differential modulation of dynein and kinesin motility suggests that MAPs can spatially regulate the balance of microtubule-dependent axonal transport.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2866193/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2866193/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dixit, Ram -- Ross, Jennifer L -- Goldman, Yale E -- Holzbaur, Erika L F -- GM-48661/GM/NIGMS NIH HHS/ -- P01 AR051174/AR/NIAMS NIH HHS/ -- P01 AR051174-050002/AR/NIAMS NIH HHS/ -- P01-AR-051174/AR/NIAMS NIH HHS/ -- R01 GM048661/GM/NIGMS NIH HHS/ -- R01 GM048661-16/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Feb 22;319(5866):1086-9. doi: 10.1126/science.1152993. Epub 2008 Jan 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18202255" target="_blank"〉PubMed〈/a〉
Keywords:
Alzheimer Disease/metabolism
;
Animals
;
Axonal Transport
;
Dyneins/chemistry/*metabolism
;
Kinesin/chemistry/*metabolism
;
Mice
;
Mice, Transgenic
;
Microscopy, Fluorescence
;
Microtubules/*metabolism
;
Models, Neurological
;
Neurons/metabolism/physiology
;
Protein Binding
;
Protein Isoforms/metabolism
;
Recombinant Fusion Proteins/metabolism
;
tau Proteins/chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
