Publication Date:
2008-02-09
Description:
The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR (ARF) proteins, which can activate transcription of auxin response genes. We show that TOPLESS (TPL) can physically interact with IAA12/BODENLOS (IAA12/BDL) through an ETHYLENE RESPONSE FACTOR (ERF)-associated amphiphilic repression (EAR) motif. TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity. In addition, tpl-1 can suppress the patterning defects of the bdl-1 mutant. Direct interaction between TPL and ARF5/MONOPTEROS, which is regulated by IAA12/BDL, results in a loss-of-function arf5/mp phenotype. These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Szemenyei, Heidi -- Hannon, Mike -- Long, Jeff A -- GM072764/GM/NIGMS NIH HHS/ -- R01 GM072764/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Mar 7;319(5868):1384-6. doi: 10.1126/science.1151461. Epub 2008 Feb 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18258861" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Arabidopsis/embryology/*genetics/metabolism
;
Arabidopsis Proteins/chemistry/genetics/*metabolism
;
DNA-Binding Proteins/metabolism
;
*Gene Expression Regulation, Plant
;
Indoleacetic Acids/*metabolism
;
Models, Genetic
;
Mutation
;
Protein Binding
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Repressor Proteins/chemistry/genetics/*metabolism
;
Seedlings/embryology/metabolism
;
Seeds/embryology/metabolism
;
Transcription Factors/metabolism
;
*Transcription, Genetic
;
Two-Hybrid System Techniques
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
