Publication Date:
2007-01-16
Description:
Environmental sequencing has revealed unimagined diversity among eukaryotic picoplankton. A distinct picoplanktonic algal group, initially detected from 18S ribosomal DNA (rDNA) sequences, was hybridized with rRNA-targeted probes, detected by tyramide signal amplification-fluorescent in situ hybridization, and showed an organelle-like body with orange fluorescence indicative of phycobilins. Using this fluorescence signal, cells were sorted by flow cytometry and probed. Hybridized cells contained a 4',6'-diamidino-2-phenylindole-stained organelle resembling a plastid with a nucleomorph. This suggests that they may be secondary endosymbiotic algae. Pending the isolation of living cells and their formal description, these algae have been termed picobiliphytes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Not, Fabrice -- Valentin, Klaus -- Romari, Khadidja -- Lovejoy, Connie -- Massana, Ramon -- Tobe, Kerstin -- Vaulot, Daniel -- Medlin, Linda K -- New York, N.Y. -- Science. 2007 Jan 12;315(5809):253-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Station Biologique de Roscoff, UMR 7144 CNRS and Universite Pierre et Marie Curie, Boite Postale 74, 29682 Roscoff Cedex, France. not@icm.csic.es〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17218530" target="_blank"〉PubMed〈/a〉
Keywords:
Bayes Theorem
;
DNA, Ribosomal/genetics
;
*Eukaryota/classification/cytology/genetics/isolation & purification
;
Flow Cytometry
;
Fluorescence
;
In Situ Hybridization, Fluorescence
;
Molecular Sequence Data
;
Organelles/ultrastructure
;
Phycobiliproteins/analysis
;
Phylogeny
;
*Phytoplankton/classification/cytology/genetics/isolation & purification
;
Seasons
;
Seawater/*microbiology
;
Symbiosis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
