Publication Date:
2006-12-13
Description:
Noncoding small RNAs regulate gene expression in all organisms, in some cases through direct association with RNA polymerase (RNAP). Here we report that the mechanism of 6S RNA inhibition of transcription is through specific, stable interactions with the active site of Escherichia coli RNAP that exclude promoter DNA binding. In fact, the DNA-dependent RNAP uses bound 6S RNA as a template for RNA synthesis, producing 14-to 20-nucleotide RNA products (pRNA). These results demonstrate that 6S RNA is functionally engaged in the active site of RNAP. Synthesis of pRNA destabilizes 6S RNA-RNAP complexes leading to release of the pRNA-6S RNA hybrid. In vivo, 6S RNA-directed RNA synthesis occurs during outgrowth from the stationary phase and likely is responsible for liberating RNAP from 6S RNA in response to nutrient availability.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wassarman, Karen M -- Saecker, Ruth M -- GM23467/GM/NIGMS NIH HHS/ -- GM67955/GM/NIGMS NIH HHS/ -- R01 GM067955/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2006 Dec 8;314(5805):1601-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA. wassarman@bact.wisc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17158328" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Binding Sites
;
DNA, Bacterial/chemistry/metabolism
;
DNA-Directed RNA Polymerases/antagonists & inhibitors/chemistry/*metabolism
;
Escherichia coli/genetics/growth & development/*metabolism
;
Molecular Sequence Data
;
Nucleic Acid Conformation
;
Promoter Regions, Genetic
;
RNA Stability
;
RNA, Bacterial/biosynthesis/chemistry/genetics/*metabolism
;
RNA, Double-Stranded/chemistry/metabolism
;
RNA, Untranslated/chemistry/genetics/*metabolism
;
Sigma Factor/*metabolism
;
Templates, Genetic
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
