Publication Date:
2007-02-03
Description:
In eukaryotes, termination of messenger RNA (mRNA) translation is mediated by the release factors eRF1 and eRF3. Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein (DBP) family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination. Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1. A physical interaction was specifically detected with eRF1. Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes. Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gross, Thomas -- Siepmann, Anja -- Sturm, Dorothee -- Windgassen, Merle -- Scarcelli, John J -- Seedorf, Matthias -- Cole, Charles N -- Krebber, Heike -- R01 GM033998/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2007 Feb 2;315(5812):646-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut fur Molekularbiologie und Tumorforschung der Philipps-Universitat Marburg, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17272721" target="_blank"〉PubMed〈/a〉
Keywords:
Codon, Terminator
;
DEAD-box RNA Helicases/genetics/*metabolism
;
Mutation
;
Nucleocytoplasmic Transport Proteins/genetics/*metabolism
;
*Peptide Chain Termination, Translational
;
Peptide Termination Factors/genetics/metabolism
;
Poly(A)-Binding Proteins/genetics/metabolism
;
Polyribosomes/metabolism
;
RNA Helicases/genetics/*metabolism
;
RNA Stability
;
RNA, Fungal/metabolism
;
RNA, Messenger/metabolism
;
Saccharomyces cerevisiae/*enzymology/*genetics
;
Saccharomyces cerevisiae Proteins/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
