Publication Date:
2006-02-18
Description:
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Giraldez, Antonio J -- Mishima, Yuichiro -- Rihel, Jason -- Grocock, Russell J -- Van Dongen, Stijn -- Inoue, Kunio -- Enright, Anton J -- Schier, Alexander F -- New York, N.Y. -- Science. 2006 Apr 7;312(5770):75-9. Epub 2006 Feb 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. giraldez@mcb.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16484454" target="_blank"〉PubMed〈/a〉
Keywords:
3' Untranslated Regions
;
Adenosine/*metabolism
;
Animals
;
Embryo, Nonmammalian/*physiology
;
*Embryonic Development
;
Female
;
Gene Expression Profiling
;
*Gene Expression Regulation, Developmental
;
Genes, Reporter
;
Green Fluorescent Proteins/genetics/metabolism
;
MicroRNAs/*physiology
;
Mutation
;
Oligonucleotide Array Sequence Analysis
;
Polymers/*metabolism
;
Protein Biosynthesis
;
RNA Stability
;
RNA, Messenger/genetics/*metabolism
;
Ribonuclease III/genetics/metabolism
;
Transcription, Genetic
;
Up-Regulation
;
Zebrafish/embryology
;
Zygote/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
