Publication Date:
2005-09-24
Description:
Energy transfer from light-harvesting carotenoids to chlorophyll is common in photosynthesis, but such antenna pigments have not been observed in retinal-based ion pumps and photoreceptors. Here we describe xanthorhodopsin, a proton-pumping retinal protein/carotenoid complex in the eubacterium Salinibacter ruber. The wavelength dependence of the rate of pumping and difference absorption spectra measured under a variety of conditions indicate that this protein contains two chromophores, retinal and the carotenoid salinixanthin, in a molar ratio of about 1:1. The two chromophores interact strongly, and light energy absorbed by the carotenoid is transferred to the retinal with a quantum efficiency of approximately 40%. The antenna carotenoid extends the wavelength range of the collection of light for uphill transmembrane proton transport.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065861/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065861/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Balashov, Sergei P -- Imasheva, Eleonora S -- Boichenko, Vladimir A -- Anton, Josefa -- Wang, Jennifer M -- Lanyi, Janos K -- GM29498/GM/NIGMS NIH HHS/ -- R37 GM029498/GM/NIGMS NIH HHS/ -- R37 GM029498-24/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2005 Sep 23;309(5743):2061-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of California, Irvine, CA 92697, USA. balashov@uci.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16179480" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Bacteroidetes/*chemistry/metabolism
;
Carotenoids/chemistry/metabolism
;
Energy Transfer
;
Glycosides/chemistry/metabolism
;
Hydrogen-Ion Concentration
;
Hydroxylamine/pharmacology
;
Light
;
Light-Harvesting Protein Complexes/*chemistry/isolation & purification/metabolism
;
Mass Spectrometry
;
Molecular Sequence Data
;
Oxygen Consumption
;
Proton Pumps/*chemistry/isolation & purification/metabolism
;
Retinaldehyde/chemistry/metabolism
;
Rhodopsins, Microbial/*chemistry/isolation & purification/metabolism
;
Spectrophotometry, Ultraviolet
;
Spectrum Analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
