Publication Date:
2005-04-09
Description:
We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be approximately 8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kural, Comert -- Kim, Hwajin -- Syed, Sheyum -- Goshima, Gohta -- Gelfand, Vladimir I -- Selvin, Paul R -- AR44420/AR/NIAMS NIH HHS/ -- GM 068625/GM/NIGMS NIH HHS/ -- GM52111/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2005 Jun 3;308(5727):1469-72. Epub 2005 Apr 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biophysics Center, University of Illinois, Urbana, IL 61801, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15817813" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Biological Transport
;
Cell Line
;
Drosophila
;
Dyneins/*physiology
;
Fluorescence
;
Green Fluorescent Proteins
;
Kinesin/*physiology
;
Molecular Motor Proteins/*physiology
;
Peroxisomes/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
