Publication Date:
2005-04-30
Description:
The clock proteins PERIOD1 (PER1) and PERIOD2 (PER2) play essential roles in a negative transcriptional feedback loop that generates circadian rhythms in mammalian cells. We identified two PER1-associated factors, NONO and WDR5, that modulate PER activity. The reduction of NONO expression by RNA interference (RNAi) attenuated circadian rhythms in mammalian cells, and fruit flies carrying a hypomorphic allele were nearly arrhythmic. WDR5, a subunit of histone methyltransferase complexes, augmented PER-mediated transcriptional repression, and its reduction by RNAi diminished circadian histone methylations at the promoter of a clock gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brown, Steven A -- Ripperger, Juergen -- Kadener, Sebastian -- Fleury-Olela, Fabienne -- Vilbois, Francis -- Rosbash, Michael -- Schibler, Ueli -- New York, N.Y. -- Science. 2005 Apr 29;308(5722):693-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology and National Centres of Competence in Research (NCCR) Frontiers in Genetics, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, CH-1211 Geneva-4, Switzerland. steven.brown@molbio.unige.ch〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15860628" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Animals
;
Carrier Proteins/genetics/*metabolism
;
Cell Cycle Proteins
;
Cell Line
;
*Circadian Rhythm
;
DNA-Binding Proteins/genetics/metabolism
;
Drosophila/genetics/physiology
;
Drosophila Proteins/genetics/physiology
;
Female
;
Gene Expression Regulation
;
Histones/metabolism
;
Immunoprecipitation
;
Male
;
Methylation
;
Mice
;
Mice, Inbred BALB C
;
Nuclear Proteins/genetics/*metabolism/physiology
;
Nuclear Receptor Subfamily 1, Group D, Member 1
;
Period Circadian Proteins
;
Promoter Regions, Genetic
;
Proteins/genetics/*metabolism
;
RNA Interference
;
Rats
;
Receptors, Cytoplasmic and Nuclear/genetics/metabolism
;
Transcription Factors
;
Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
