Publication Date:
2005-02-05
Description:
Plants encode subunits for a fourth RNA polymerase (Pol IV) in addition to the well-known DNA-dependent RNA polymerases I, II, and III. By mutation of the two largest subunits (NRPD1a and NRPD2), we show that Pol IV silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3. The existence of this distinct silencing polymerase may explain the paradoxical involvement of an RNA silencing pathway in maintenance of transcriptional silencing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Herr, A J -- Jensen, M B -- Dalmay, T -- Baulcombe, D C -- New York, N.Y. -- Science. 2005 Apr 1;308(5718):118-20. Epub 2005 Feb 3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15692015" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Arabidopsis/*enzymology/genetics
;
Arabidopsis Proteins/chemistry/genetics/metabolism
;
Base Sequence
;
Chromatin/metabolism
;
DNA Methylation
;
DNA Transposable Elements
;
DNA, Plant/*genetics/metabolism
;
DNA-Directed RNA Polymerases/chemistry/genetics/*metabolism
;
*Gene Silencing
;
Genes, Plant
;
Genetic Complementation Test
;
Green Fluorescent Proteins/genetics/metabolism
;
Molecular Sequence Data
;
Mutation
;
Oryza/enzymology/genetics
;
Plants, Genetically Modified
;
Protein Subunits/chemistry/genetics/metabolism
;
RNA Interference
;
RNA Polymerase II/metabolism
;
RNA, Plant/metabolism
;
RNA, Small Interfering/metabolism
;
Repetitive Sequences, Nucleic Acid
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
