Publication Date:
2005-01-08
Description:
Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5'-triphosphate (GTP) analog GTPgS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yamashita, Takayuki -- Hige, Toshihide -- Takahashi, Tomoyuki -- New York, N.Y. -- Science. 2005 Jan 7;307(5706):124-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurophysiology, University of Tokyo Graduate School of Medicine, Tokyo 113-0033, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15637282" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Botulinum Toxins/metabolism
;
Brain Stem/*metabolism
;
Calcium/metabolism
;
Dynamin I/pharmacology/*physiology
;
Electric Capacitance
;
*Endocytosis
;
Excitatory Postsynaptic Potentials
;
Exocytosis
;
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
;
Guanosine Diphosphate/*analogs & derivatives/pharmacology
;
Guanosine Triphosphate/*metabolism
;
Hydrolysis
;
In Vitro Techniques
;
Patch-Clamp Techniques
;
Peptide Fragments/pharmacology
;
Rats
;
Rats, Wistar
;
Synapses/*physiology
;
Synaptic Transmission
;
Synaptic Vesicles/*metabolism
;
Thionucleotides/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
