Publikationsdatum:
2004-11-06
Beschreibung:
Type III secretion systems (TTSSs) mediate translocation of virulence factors into host cells. We report the 17-angstrom resolution structures of a central component of Salmonella typhimurium TTSS, the needle complex, and its assembly precursor, the bacterial envelope-anchored base. Both the base and the fully assembled needle complex adopted multiple oligomeric states in vivo, and needle assembly was accompanied by recruitment of the protein PrgJ as a structural component of the base. Moreover, conformational changes during needle assembly created scaffolds for anchoring both PrgJ and the needle substructure and may provide the basis for substrate-specificity switching during type III secretion.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1459965/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1459965/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marlovits, Thomas C -- Kubori, Tomoko -- Sukhan, Anand -- Thomas, Dennis R -- Galan, Jorge E -- Unger, Vinzenz M -- AI30492/AI/NIAID NIH HHS/ -- GM35433/GM/NIGMS NIH HHS/ -- GM66145/GM/NIGMS NIH HHS/ -- P42 RR-01081/RR/NCRR NIH HHS/ -- R01 GM066145/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Nov 5;306(5698):1040-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520-8024, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15528446" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Bacterial Proteins/chemistry/*ultrastructure
;
Cryoelectron Microscopy
;
Macromolecular Substances
;
Membrane Transport Proteins/chemistry/ultrastructure
;
Salmonella typhimurium/chemistry/*ultrastructure
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
