Publication Date:
2004-08-07
Description:
The cascade of events that leads to vaccinia-induced actin polymerization requires Src-dependent tyrosine phosphorylation of the viral membrane protein A36R. We found that a localized outside-in signaling cascade induced by the viral membrane protein B5R is required to potently activate Src and induce A36R phosphorylation at the plasma membrane. In addition, Src-mediated phosphorylation of A36R regulated the ability of virus particles to recruit and release conventional kinesin. Thus, Src activity regulates the transition between cytoplasmic microtubule transport and actin-based motility at the plasma membrane.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Newsome, Timothy P -- Scaplehorn, Niki -- Way, Michael -- New York, N.Y. -- Science. 2004 Oct 1;306(5693):124-9. Epub 2004 Aug 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell Motility Laboratory, Room 529, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15297625" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/*metabolism
;
Animals
;
Cell Line
;
Cell Membrane/metabolism/virology
;
Chickens
;
Consensus Sequence
;
Enzyme Activation
;
HeLa Cells
;
Humans
;
Kinesin/metabolism
;
Membrane Glycoproteins/chemistry/metabolism
;
Microtubules/*metabolism
;
Phosphorylation
;
Phosphotyrosine/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Vaccinia virus/genetics/*metabolism/physiology
;
Viral Envelope Proteins/chemistry/metabolism
;
Viral Structural Proteins/*metabolism
;
Virion/metabolism
;
src-Family Kinases/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
