Publication Date:
2004-10-23
Description:
We used a maskless photolithography method to produce DNA oligonucleotide microarrays with unique probe sequences tiled throughout the genome of Drosophila melanogaster and across predicted splice junctions. RNA expression of protein coding and nonprotein coding sequences was determined for each major stage of the life cycle, including adult males and females. We detected transcriptional activity for 93% of annotated genes and RNA expression for 41% of the probes in intronic and intergenic sequences. Comparison to genome-wide RNA interference data and to gene annotations revealed distinguishable levels of expression for different classes of genes and higher levels of expression for genes with essential cellular functions. Differential splicing was observed in about 40% of predicted genes, and 5440 previously unknown splice forms were detected. Genes within conserved regions of synteny with D. pseudoobscura had highly correlated expression; these regions ranged in length from 10 to 900 kilobase pairs. The expressed intergenic and intronic sequences are more likely to be evolutionarily conserved than nonexpressed ones, and about 15% of them appear to be developmentally regulated. Our results provide a draft expression map for the entire nonrepetitive genome, which reveals a much more extensive and diverse set of expressed sequences than was previously predicted.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stolc, Viktor -- Gauhar, Zareen -- Mason, Christopher -- Halasz, Gabor -- van Batenburg, Marinus F -- Rifkin, Scott A -- Hua, Sujun -- Herreman, Tine -- Tongprasit, Waraporn -- Barbano, Paolo Emilio -- Bussemaker, Harmen J -- White, Kevin P -- P20LM007276-01/LM/NLM NIH HHS/ -- T32GM008224-17/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Oct 22;306(5696):655-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15499012" target="_blank"〉PubMed〈/a〉
Keywords:
Algorithms
;
Animals
;
Computational Biology
;
DNA, Intergenic
;
Drosophila/genetics
;
Drosophila Proteins/genetics/physiology
;
Drosophila melanogaster/*genetics/growth & development
;
Evolution, Molecular
;
Exons
;
Female
;
*Gene Expression
;
*Gene Expression Profiling
;
Genes, Insect
;
*Genome
;
Introns
;
Life Cycle Stages
;
Male
;
Oligonucleotide Array Sequence Analysis
;
Oligonucleotide Probes
;
RNA Splicing
;
Synteny
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
