Publication Date:
2004-06-19
Description:
Salmonella enterica, the cause of food poisoning and typhoid fever, induces actin cytoskeleton rearrangements and membrane ruffling to gain access into nonphagocytic cells, where it can replicate and avoid innate immune defenses. Here, we found that SopB, a phosphoinositide phosphatase that is delivered into host cells by a type III secretion system, was essential for the establishment of Salmonella's intracellular replicative niche. SopB mediated the formation of spacious phagosomes following bacterial entry and was responsible for maintaining high levels of phosphatidylinositol-three-phosphate [PtdIns(3)P] in the membrane of the bacteria-containing vacuoles. Absence of SopB caused a significant defect in the maturation of the Salmonella-containing vacuole and impaired bacterial intracellular growth.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hernandez, Lorraine D -- Hueffer, Karsten -- Wenk, Markus R -- Galan, Jorge E -- AI055472/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2004 Jun 18;304(5678):1805-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15205533" target="_blank"〉PubMed〈/a〉
Keywords:
Antigens, CD/metabolism
;
Bacterial Proteins/genetics/*metabolism
;
Cell Line
;
Cell Membrane/metabolism/ultrastructure
;
Cytoplasmic Vesicles/metabolism/*microbiology/ultrastructure
;
Epithelial Cells/microbiology
;
Gene Deletion
;
Genomic Islands
;
Humans
;
Intestinal Mucosa/cytology/*microbiology
;
Lysosome-Associated Membrane Glycoproteins
;
Microscopy, Video
;
Mutation
;
Phagosomes/metabolism/*microbiology
;
Phosphatidylinositol Phosphates/metabolism
;
Phosphatidylinositols/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Salmonella typhimurium/genetics/growth & development/*metabolism/pathogenicity
;
Vacuoles/metabolism/microbiology/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
