Publication Date:
2004-04-06
Description:
Because ethical restrictions limit in vivo studies of the human hemato-lymphoid system, substitute human to small animal xenotransplantation models have been employed. Existing models, however, sustain only limited development and maintenance of human lymphoid cells and rarely produce immune responses. Here we show that intrahepatic injection of CD34+ human cord blood cells into conditioned newborn Rag2-/-gammac-/- mice leads to de novo development of B, T, and dendritic cells; formation of structured primary and secondary lymphoid organs; and production of functional immune responses. This provides a valuable model to study development and function of the human adaptive immune system in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Traggiai, Elisabetta -- Chicha, Laurie -- Mazzucchelli, Luca -- Bronz, Lucio -- Piffaretti, Jean-Claude -- Lanzavecchia, Antonio -- Manz, Markus G -- New York, N.Y. -- Science. 2004 Apr 2;304(5667):104-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Research in Biomedicine (IRB), Via Vela 6, 6500 Bellinzona, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15064419" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Newborn
;
Antigens, CD34/analysis
;
B-Lymphocytes/cytology/*immunology
;
Bone Marrow Cells/immunology
;
Dendritic Cells/cytology/*immunology
;
Dendritic Cells, Follicular/cytology/immunology
;
Epstein-Barr Virus Infections/immunology
;
Fetal Blood/*cytology
;
Gene Rearrangement, T-Lymphocyte
;
*Hematopoietic Stem Cell Transplantation
;
Humans
;
Immune System/*physiology
;
Immunoglobulins/analysis
;
Infant, Newborn
;
Lymph Nodes/cytology/immunology
;
Lymphocyte Activation
;
Major Histocompatibility Complex
;
Mice
;
Mice, Inbred BALB C
;
Mice, Mutant Strains
;
Spleen/cytology/immunology
;
T-Lymphocytes/cytology/*immunology
;
Tetanus Toxoid/immunology
;
Thymus Gland/cytology/immunology
;
Transplantation, Heterologous
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
