Publication Date:
2004-01-13
Description:
The anaphase-promoting complex (APC) is highly expressed in postmitotic neurons, but its function in the nervous system was previously unknown. We report that the inhibition of Cdh1-APC in primary neurons specifically enhanced axonal growth. Cdh1 knockdown in cerebellar slice overlay assays and in the developing rat cerebellum in vivo revealed cell-autonomous abnormalities in layer-specific growth of granule neuron axons and parallel fiber patterning. Cdh1 RNA interference in neurons was also found to override the inhibitory influence of myelin on axonal growth. Thus, Cdh1-APC appears to play a role in regulating axonal growth and patterning in the developing brain that may also limit the growth of injured axons in the adult brain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Konishi, Yoshiyuki -- Stegmuller, Judith -- Matsuda, Takahiko -- Bonni, Shirin -- Bonni, Azad -- R01NS41021/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2004 Feb 13;303(5660):1026-30. Epub 2004 Jan 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14716021" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase-Promoting Complex-Cyclosome
;
Animals
;
Axons/*physiology/ultrastructure
;
Cell Cycle
;
Cell Cycle Proteins/metabolism
;
Cell Nucleus/metabolism
;
Cells, Cultured
;
Cerebellar Cortex/*cytology/growth & development
;
Dendrites/physiology/ultrastructure
;
Electroporation
;
Morphogenesis
;
Mutation
;
Myelin Sheath/metabolism
;
Neurons/*physiology
;
Organ Culture Techniques
;
RNA Interference
;
Rats
;
Rats, Long-Evans
;
Transfection
;
Ubiquitin-Protein Ligase Complexes/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
