Publication Date:
2003-10-18
Description:
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dahan, Maxime -- Levi, Sabine -- Luccardini, Camilla -- Rostaing, Philippe -- Riveau, Beatrice -- Triller, Antoine -- New York, N.Y. -- Science. 2003 Oct 17;302(5644):442-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Superieure and Universite Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France. maxime.dahan@lkb.ens.fr〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14564008" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Membrane/metabolism/ultrastructure
;
Cells, Cultured
;
Dendrites/metabolism/ultrastructure
;
Diffusion
;
Fluorescence
;
*Fluorescent Dyes
;
Microscopy, Electron
;
*Nanotechnology
;
Neurites/metabolism/ultrastructure
;
Neurons/*metabolism/ultrastructure
;
Pyridinium Compounds
;
Quaternary Ammonium Compounds
;
Rats
;
Rats, Sprague-Dawley
;
Receptors, Glycine/*metabolism
;
Semiconductors
;
Spinal Cord/cytology
;
Synapses/metabolism/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
