Publication Date:
2002-12-21
Description:
ACT is a LIM-only protein expressed exclusively in round spermatids, where it cooperates with transcriptional activator CREM in regulating various postmeiotic genes. Targeted inactivation of CREM leads to a complete block of mouse spermiogenesis. We sought to identify the regulatory steps controlling the functional interplay between CREM and ACT. We found that ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells. The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT. Sensitivity to leptomycin B indicates that KIF17b can be actively exported from the nucleus through the Crm1 receptor. Thus, a kinesin directly controls the activity of a transcriptional coactivator by a tight regulation of its intracellular localization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Macho, Betina -- Brancorsini, Stefano -- Fimia, Gian Maria -- Setou, Mitsutoshi -- Hirokawa, Nobutaka -- Sassone-Corsi, Paolo -- New York, N.Y. -- Science. 2002 Dec 20;298(5602):2388-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut de Genetique et de Biologie Moleculaire et Cellulaire, B. P. 10142, 67404 Illkirch, Strasbourg, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12493914" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Amino Acid Sequence
;
Animals
;
COS Cells
;
Cell Nucleus/metabolism
;
Cyclic AMP Response Element Modulator
;
Cytoplasm/metabolism
;
DNA-Binding Proteins/metabolism
;
Fatty Acids, Unsaturated/pharmacology
;
Gene Expression Regulation, Developmental
;
Karyopherins/metabolism
;
Kinesin/chemistry/genetics/*metabolism
;
LIM Domain Proteins
;
Male
;
Mice
;
Molecular Motor Proteins/chemistry/genetics/*metabolism
;
Molecular Sequence Data
;
Promoter Regions, Genetic
;
Protein Isoforms/chemistry/genetics/metabolism
;
*Receptors, Cytoplasmic and Nuclear
;
*Repressor Proteins
;
Spermatids/*metabolism
;
*Spermatogenesis
;
Testis/metabolism
;
Transcription Factors/genetics/*metabolism
;
*Transcription, Genetic
;
Transcriptional Activation
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
