Publication Date:
2002-08-17
Description:
COP9 signalosome (CSN) cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. The Jab1/MPN domain metalloenzyme (JAMM) motif in the Jab1/Csn5 subunit was found to underlie CSN's Nedd8 isopeptidase activity. JAMM is found in proteins from archaea, bacteria, and eukaryotes, including the Rpn11 subunit of the 26S proteasome. Metal chelators and point mutations within JAMM abolished CSN-dependent cleavage of Nedd8 from Cul1, yet had little effect on CSN complex assembly. Optimal SCF activity in yeast and both viability and proper photoreceptor cell (R cell) development in Drosophila melanogaster required an intact Csn5 JAMM domain. We propose that JAMM isopeptidases play important roles in a variety of physiological pathways.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cope, Gregory A -- Suh, Greg S B -- Aravind, L -- Schwarz, Sylvia E -- Zipursky, S Lawrence -- Koonin, Eugene V -- Deshaies, Raymond J -- New York, N.Y. -- Science. 2002 Oct 18;298(5593):608-11. Epub 2002 Aug 15.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, California Institute of Technology (CalTech), Pasadena, CA 91125, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12183637" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Animals
;
Carbon-Nitrogen Lyases/metabolism
;
Cell Cycle Proteins/*metabolism
;
*Cullin Proteins
;
DNA-Binding Proteins/*chemistry/*metabolism
;
Drosophila Proteins
;
Drosophila melanogaster/growth & development/metabolism
;
Humans
;
Intracellular Signaling Peptides and Proteins
;
Metalloendopeptidases/*chemistry/genetics/*metabolism
;
Molecular Sequence Data
;
Mutation
;
Peptide Hydrolases
;
Peptide Synthases/metabolism
;
SKP Cullin F-Box Protein Ligases
;
Saccharomyces cerevisiae/metabolism
;
Saccharomyces cerevisiae Proteins/chemistry/genetics/metabolism
;
Schizosaccharomyces/genetics/metabolism
;
Sequence Alignment
;
Temperature
;
Transcription Factors/*chemistry/*metabolism
;
Ubiquitins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
