Publication Date:
2002-08-10
Description:
Origin recognition complex (ORC) proteins serve as a landing pad for the assembly of a multiprotein prereplicative complex, which is required to initiate DNA replication. During mitosis, the smallest subunit of human ORC, Orc6, localizes to kinetochores and to a reticular-like structure around the cell periphery. As chromosomes segregate during anaphase, the reticular structures align along the plane of cell division and some Orc6 localizes to the midbody before cells separate. Silencing of Orc6 expression by small interfering RNA (siRNA) resulted in cells with multipolar spindles, aberrant mitosis, formation of multinucleated cells, and decreased DNA replication. Prolonged periods of Orc6 depletion caused a decrease in cell proliferation and increased cell death. These results implicate Orc6 as an essential gene that coordinates chromosome replication and segregation with cytokinesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prasanth, Supriya G -- Prasanth, Kannanganattu V -- Stillman, Bruce -- CA13106/CA/NCI NIH HHS/ -- P01 CA013106/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 9;297(5583):1026-31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12169736" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bromodeoxyuridine/metabolism
;
Cell Death
;
*Cell Division
;
Cell Line
;
Cell Nucleus/metabolism
;
Cells, Cultured
;
Centromere/metabolism
;
*Chromosome Segregation
;
Chromosomes, Human/*metabolism
;
*DNA Replication
;
DNA-Binding Proteins/genetics/metabolism/*physiology
;
Fluorescent Antibody Technique
;
Gene Silencing
;
Humans
;
Kinetochores/metabolism
;
Mitosis
;
Origin Recognition Complex
;
Phenotype
;
Polyploidy
;
RNA, Small Interfering
;
RNA, Untranslated/metabolism/pharmacology
;
Recombinant Fusion Proteins/analysis
;
Saccharomyces cerevisiae Proteins
;
Spindle Apparatus/ultrastructure
;
Transfection
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
