Publication Date:
2002-04-06
Description:
Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stevens, Jennitte L -- Cantin, Greg T -- Wang, Gang -- Shevchenko, Andrej -- Shevchenko, Anna -- Berk, Arnold J -- CA25235/CA/NCI NIH HHS/ -- GM07185/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Apr 26;296(5568):755-8. Epub 2002 Apr 4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11934987" target="_blank"〉PubMed〈/a〉
Keywords:
Adenovirus E1A Proteins/*metabolism
;
Adenoviruses, Human/physiology
;
Animals
;
Carrier Proteins/genetics/*metabolism
;
Cells, Cultured
;
*DNA-Binding Proteins
;
Genes, Immediate-Early
;
HeLa Cells
;
Humans
;
*MAP Kinase Signaling System
;
Mediator Complex
;
Mice
;
Mice, Knockout
;
Mitogen-Activated Protein Kinases/metabolism
;
Mutation
;
Promoter Regions, Genetic
;
Proto-Oncogene Proteins/metabolism
;
Stem Cells/*metabolism
;
*Trans-Activators
;
Transcription Factors/metabolism
;
*Transcription, Genetic
;
*Transcriptional Activation
;
Transfection
;
ets-Domain Protein Elk-1
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
