Publication Date:
2001-04-09
Description:
The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates longevity and stress resistance in Caenorhabditis elegans. We screened for long-lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fabrizio, P -- Pozza, F -- Pletcher, S D -- Gendron, C M -- Longo, V D -- AG 08761-10/AG/NIA NIH HHS/ -- AG09793/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 2001 Apr 13;292(5515):288-90. Epub 2001 Apr 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biogerontology, Andrus Gerontology Center, and Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0191, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11292860" target="_blank"〉PubMed〈/a〉
Keywords:
Adenylyl Cyclases/genetics/metabolism
;
Amino Acid Sequence
;
Animals
;
Caenorhabditis elegans/genetics/physiology
;
Culture Media
;
Cyclic AMP-Dependent Protein Kinases/metabolism
;
DNA Transposable Elements
;
DNA-Binding Proteins/genetics/metabolism
;
Drosophila/genetics/physiology
;
Drug Resistance, Microbial
;
Gene Deletion
;
Hot Temperature
;
Longevity
;
Molecular Sequence Data
;
Mutagenesis, Insertional
;
Oxidants/pharmacology
;
Paraquat/pharmacology
;
Phenotype
;
Protein Kinases/chemistry/genetics/*metabolism
;
Saccharomyces cerevisiae/enzymology/genetics/*physiology
;
*Saccharomyces cerevisiae Proteins
;
Signal Transduction
;
Transcription Factors/genetics/metabolism
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
