Publication Date:
2001-02-07
Description:
Replicative senescence is thought to be an intrinsic mechanism for limiting the proliferative life-span of normal somatic cells. We show here that rat Schwann cells can be expanded indefinitely in culture while maintaining checkpoints normally lost during the immortalization process. These findings demonstrate that senescence is not an inevitable consequence of extended proliferation in culture.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mathon, N F -- Malcolm, D S -- Harrisingh, M C -- Cheng, L -- Lloyd, A C -- New York, N.Y. -- Science. 2001 Feb 2;291(5505):872-5. Epub 2001 Jan 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11157166" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blood
;
Carrier Proteins/metabolism
;
*Cell Aging
;
Cell Culture Techniques
;
*Cell Division
;
Cell Line
;
Cell Size
;
Cells, Cultured
;
Clone Cells
;
Culture Media
;
Cyclin-Dependent Kinase Inhibitor p16
;
Cyclin-Dependent Kinases/metabolism
;
Cyclins/metabolism
;
Fibroblasts/cytology/physiology
;
Giant Cells/cytology
;
Mutation
;
Phenotype
;
Proteins/metabolism
;
Rats
;
Schwann Cells/*cytology/physiology
;
Telomerase/metabolism
;
Telomere/physiology
;
Tumor Suppressor Protein p14ARF
;
Tumor Suppressor Protein p53/metabolism
;
beta-Galactosidase/metabolism
;
ras Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
