Publication Date:
2012-02-24
Description:
Packaging of proteins from the endoplasmic reticulum into COPII vesicles is essential for secretion. In cells, most COPII vesicles are approximately 60-80 nm in diameter, yet some must increase their size to accommodate 300-400 nm procollagen fibres or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII coats have remained elusive. Here, we identified the ubiquitin ligase CUL3-KLHL12 as a regulator of COPII coat formation. CUL3-KLHL12 catalyses the monoubiquitylation of the COPII-component SEC31 and drives the assembly of large COPII coats. As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292188/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292188/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jin, Lingyan -- Pahuja, Kanika Bajaj -- Wickliffe, Katherine E -- Gorur, Amita -- Baumgartel, Christine -- Schekman, Randy -- Rape, Michael -- DP2 OD003088/OD/NIH HHS/ -- DP2 OD003088-01/OD/NIH HHS/ -- R01 GM083064/GM/NIGMS NIH HHS/ -- R01 GM083064-04/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2012 Feb 22;482(7386):495-500. doi: 10.1038/nature10822.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22358839" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
COP-Coated Vesicles/*chemistry/*metabolism
;
Cell Line
;
Cell Shape
;
Collagen/metabolism
;
Cullin Proteins/genetics/metabolism
;
Embryonic Stem Cells/cytology/metabolism
;
HeLa Cells
;
Humans
;
Mice
;
Microfilament Proteins/genetics/metabolism
;
Protein Transport
;
Ubiquitin/*metabolism
;
Ubiquitination
;
Vesicular Transport Proteins/genetics/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
