Publication Date:
2012-02-22
Description:
Mechanotransduction has an important role in physiology. Biological processes including sensing touch and sound waves require as-yet-unidentified cation channels that detect pressure. Mouse Piezo1 (MmPiezo1) and MmPiezo2 (also called Fam38a and Fam38b, respectively) induce mechanically activated cationic currents in cells; however, it is unknown whether Piezo proteins are pore-forming ion channels or modulate ion channels. Here we show that Drosophila melanogaster Piezo (DmPiezo, also called CG8486) also induces mechanically activated currents in cells, but through channels with remarkably distinct pore properties including sensitivity to the pore blocker ruthenium red and single channel conductances. MmPiezo1 assembles as a approximately 1.2-million-dalton homo-oligomer, with no evidence of other proteins in this complex. Purified MmPiezo1 reconstituted into asymmetric lipid bilayers and liposomes forms ruthenium-red-sensitive ion channels. These data demonstrate that Piezo proteins are an evolutionarily conserved ion channel family involved in mechanotransduction.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297710/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297710/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Coste, Bertrand -- Xiao, Bailong -- Santos, Jose S -- Syeda, Ruhma -- Grandl, Jorg -- Spencer, Kathryn S -- Kim, Sung Eun -- Schmidt, Manuela -- Mathur, Jayanti -- Dubin, Adrienne E -- Montal, Mauricio -- Patapoutian, Ardem -- R01 DE022115/DE/NIDCR NIH HHS/ -- R01 DE022115-01/DE/NIDCR NIH HHS/ -- R01 DE022115-02/DE/NIDCR NIH HHS/ -- R01 GM049711/GM/NIGMS NIH HHS/ -- R01 NS046303/NS/NINDS NIH HHS/ -- R01 NS046303-09/NS/NINDS NIH HHS/ -- England -- Nature. 2012 Feb 19;483(7388):176-81. doi: 10.1038/nature10812.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22343900" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Drosophila Proteins/chemistry/genetics/metabolism
;
Drosophila melanogaster
;
Electric Conductivity
;
HEK293 Cells
;
HeLa Cells
;
Humans
;
*Ion Channel Gating
;
Ion Channels/*chemistry/genetics/*metabolism
;
Lipid Bilayers/chemistry/metabolism
;
Mechanotransduction, Cellular/*physiology
;
Mice
;
Molecular Sequence Data
;
NIH 3T3 Cells
;
Porosity
;
Protein Multimerization
;
Protein Subunits/chemistry/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
