Publikationsdatum:
2011-02-07
Beschreibung:
A specific and sensitive reverse transcriptase-nested polymerase chain reaction assay (RT-nPCR) was developed for the detection of Citrus tristeza virus (CTV) from naturally infected citrus samples. Two sets of primer pairs were designed by alignment of nucleotide sequences available in GenBank database for different genotypes of CTV. RT-nPCR reaction components and thermal cycling parameters were optimized and reaction conditions were standardized. Sequencing of the PCR products from direct and nested-PCR reactions confirmed the specificity of both primer pairs. Presence of CTV specific amplicons in asymptomatic samples which were collected from diseased orchards indicated the sensitivity of the test. As RT-nPCR technique, developed in the present study, is specific and efficient in detecting CTV, this could be envisioned for diagnostic applications and surveillance. Content Type Journal Article Pages 1-5 DOI 10.1007/s00284-011-9883-7 Authors Charith Raj Adkar-Purushothama, Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, University of Mysore, Manasagangothri, Mysore, 570 006 Karnataka India P. K. Maheshwar, Department of Microbiology, Yuvaraja’s College, University of Mysore, Mysore, 570 005 Karnataka India Teruo Sano, Laboratory of Plant Pathology, Faculty of Agriculture and Life Sciences, Hirosaki University, Hirosaki, Japan G. R. Janardhana, Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, University of Mysore, Manasagangothri, Mysore, 570 006 Karnataka India Journal Current Microbiology Online ISSN 1432-0991 Print ISSN 0343-8651
Print ISSN:
0343-8651
Digitale ISSN:
1432-0991
Thema:
Biologie
,
Medizin
