Publication Date:
2016-01-22
Description:
Article CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci. Nature Communications doi: 10.1038/ncomms10431 Authors: Kazuto Yoshimi, Yayoi Kunihiro, Takehito Kaneko, Hitoshi Nagahora, Birger Voigt, Tomoji Mashimo
Electronic ISSN:
2041-1723
Topics:
Biology
,
Chemistry and Pharmacology
,
Natural Sciences in General
,
Physics