Publication Date:
2012-09-15
Description:
Fourier-transform infrared spectroscopy and non-isothermal methods—chemiluminometry, differential scanning calorimetry, and differential thermogravimetry—were used to characterize potential structural changes of thiol-modified hyaluronans. Degradative conditions tested via rotational viscometry were first initiated applying oxidative Weissberger ’s system in a reaction system under aerobic conditions. Several low-molecular-weight thiol compounds—cysteamine, l -cysteine, and N -acetyl- l -cysteine—were subsequently tested for their potential antioxidative effects against hyaluronan degradation. It was shown that different final values of dynamic viscosity of hyaluronan solutions were dependent on the thiol structure and its initial concentration. An idea has been put forward that together with the reduction of the hyaluronan molecular weight, which is a consequence of fragmentation, the degradation products might contain associated or even cross-linked structures. In the case of N -acetyl- l -cysteine application, the carbonaceous residue evidenced by differential thermogravimetry was increased when compared to that of intact hyaluronan. Content Type Journal Article Category Original Paper Pages 1-12 DOI 10.1007/s10570-012-9775-4 Authors Eva Hrabárová, Department of Carbohydrate Enzymology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic Jozef Rychlý, Polymer Institute, Slovak Academy of Sciences, 84541 Bratislava, Slovak Republic Vlasta Sasinková, Department of Analytical Chemistry, Institute of Chemistry, Slovak Academy of Sciences, 84538 Bratislava, Slovak Republic Katarína Valachová, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 84104 Bratislava, Slovak Republic Ivica Janigová, Polymer Institute, Slovak Academy of Sciences, 84541 Bratislava, Slovak Republic Katarína Csomorová, Polymer Institute, Slovak Academy of Sciences, 84541 Bratislava, Slovak Republic Ivo Juránek, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 84104 Bratislava, Slovak Republic Ladislav Šoltés, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 84104 Bratislava, Slovak Republic Journal Cellulose Online ISSN 1572-882X Print ISSN 0969-0239
Print ISSN:
0969-0239
Electronic ISSN:
1572-882X
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
