Publication Date:
2012-06-20
Description:
Lipase (Lipozyme TL IM)-mediated biocatalysis in a solvent-free system was effectively employed to convert coconut oil and fusel oil to flavor-active octanoic acid esters (mainly ethyl-, butyl-, isobutyl- and (iso)amyl octanoate). Response surface methodology based on five-level-three-factor central composite design was applied to investigate the effects of single and multiple interactive factors on the conversion. Results indicated that the studied independent parameters including reactant molar ratio, enzyme loading and shaking speed were all significant factors that affected the formation of octanoic acid esters during transesterification reaction. According to the developed polynomial model ( R 2 = 0.947), the optimum transesterification conditions were determined as follows: molar ratio of 3.25:1 (alcohol/oil), enzyme loading of 16.7 % (wt%) and shaking speed of 160 rpm. Under the optimized reaction conditions an average conversion of 62.0 % was achieved which was in reasonable agreement with the predicted value of 64.1 % and about 9.4 mg of ethyl octanoate, 14.4 mg of isobutyl octanoate, 0.3 mg of butyl octanoate and 49.2 mg of (iso)amyl octanoate were produced per gram of coconut oil. Content Type Journal Article Category Original Paper Pages 1-8 DOI 10.1007/s11746-012-2099-6 Authors Jingcan Sun, Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Singapore Jin Hua Chin, Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Singapore Weibiao Zhou, Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Singapore Bin Yu, Firmenich Asia Pte Ltd, Tuas, Singapore, Singapore Philip Curran, Firmenich Asia Pte Ltd, Tuas, Singapore, Singapore Shao-Quan Liu, Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Singapore Journal Journal of the American Oil Chemists' Society Online ISSN 1558-9331 Print ISSN 0003-021X
Print ISSN:
0003-021X
Electronic ISSN:
1558-9331
Topics:
Chemistry and Pharmacology
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Process Engineering, Biotechnology, Nutrition Technology
