Publikationsdatum:
2020
Beschreibung:
〈p〉Publication date: March 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 98〈/p〉
〈p〉Author(s): Sheng-Wei Luo, Zi-Ye Luo, Teng Yan, Kai-Kun Luo, Ping-Hui Feng, Shao-Jun Liu〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Hepcidin, a multifunctional hormone oligopeptide, not only exhibits a regulatory role in iron metabolism, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-hepcidin was 258 bp and encoded 85 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-hepcidin was observed in liver. 〈em〉Aeromonas hydrophila〈/em〉 challenge can sharply increased WR-hepcidin mRNA expression in liver, trunk kidney and spleen. The purified WR-hepcidin fusion peptide can directly bind to 〈em〉A. hydrophila〈/em〉 and 〈em〉Streptococcus agalactiae〈/em〉, reduce the relative bacterial activity, limit bacterial growth and attenuate their dissemination to tissues 〈em〉in vivo〈/em〉. In addition, the treatment of WR-hepcidin fusion protein can diminish the production of pro-inflammatory cytokines. These results indicated that WR-hepcidin can play a negative regulatory role in bacteria-stimulated pro-inflammatory cytokines production and MyD88-IRAK4 activation.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Digitale ISSN:
1095-9947
Thema:
Biologie
,
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
,
Medizin
