Publication Date:
2020
Description:
〈p〉Publication date: March 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 98〈/p〉
〈p〉Author(s): Yan-li Su, Guang-hua Wang, Jing-jing Wang, Bing Xie, Qin-qin Gu, Dong-fang Hao, Hong-mei Liu, Min Zhang〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉At present, several reports have indicated that the C-terminal peptides of tissue factor pathway inhibitor 1 (TFPI-1) were active antibacterial peptides. However, the functions of TFPI-1 C-terminal peptides in teleost are still very limited. In this study, a C-terminal peptide, TC26 (with 26 amino acids), derived from common carp (〈em〉Cyprinus carpio〈/em〉) TFPI-1, was synthesized and investigated for its antibacterial spectrum, action mechanism, as well as the 〈em〉in vivo〈/em〉 effects on bacterial invasion. Our results showed that TC26 was active against Gram-positive bacteria 〈em〉Micrococcus luteus〈/em〉 and 〈em〉Staphylococcus aureus〈/em〉, as well as Gram-negative bacterium 〈em〉Vibrio vulnificus〈/em〉. TC26 treatment facilitated the bactericidal process of erythromycin by enhancing the out-membrane permeability of 〈em〉V. vulnificus〈/em〉. During the bactericidal process, TC26 killed the target bacterial cells 〈em〉Vibrio vulnificus〈/em〉, by destroying cell membrane integrity, penetrating into the cytoplasm and inducing degradation of genomic DNA and total RNA. 〈em〉In vivo〈/em〉 study showed that administration of turbot with TC26 before bacterial infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC26 is a novel and active antibacterial peptide and may play a vital role in fighting pathogenic infection in aquaculture.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
