Publikationsdatum:
2020
Beschreibung:
〈p〉Publication date: March 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 98〈/p〉
〈p〉Author(s): Ying-Yu Tang, Qiu-Ning Liu, Cheng Wang, Ting-Ting Yang, Bo-Ping Tang, Chun-Lin Zhou, Li-Shang Dai〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉〈em〉Procambarus clarkii〈/em〉 is one of the most important aquatic invertebrates in China and has high commercial value. However, aquaculture has suffered great economic loss due to outbreaks of infectious diseases in 〈em〉P〈/em〉. 〈em〉clarkii〈/em〉. To identify red swamp crayfish related proteins involved in the response to bacterial infection, we analysed immune-related proteins following lipopolysaccharide (LPS) stimulation by quantitative proteomics. The proteome of the hepatopancreas of 〈em〉P〈/em〉. 〈em〉clarkii〈/em〉 challenged with LPS and phosphate-buffered saline was analysed to evaluate the immune response. Based on liquid chromatography coupled with tandem mass spectrometry, 16 upregulated and 29 downregulated proteins were identified. A Gene Ontology analysis demonstrated 5 biological process, 11 cellular component, and 6 molecular function subcategories. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the identified proteins were mainly involved in metabolism, phagosome, and ribosome. Real-time quantitative reverse transcription-PCR revealed that eight immune-related genes were upregulated after LPS stimulation compared to the control. Taken together, the data enhance our understanding of the immune response of crayfish to LPS.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Digitale ISSN:
1095-9947
Thema:
Biologie
,
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
,
Medizin
