ALBERT

Beschreibung: 〈p〉Publication date: March 2020〈/p〉 〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 98〈/p〉 〈p〉Author(s): Junjie Nie, Zhixue Yu, Defu Yao, Fan Wang, Chunhua Zhu, Kaihui Sun, Jude Juventus Aweya, Yueling Zhang〈/p〉 〈div xml:lang="en"〉 〈h5〉Abstract〈/h5〉 〈div〉〈p〉The histone deacetylase, sirtuin 6 (SIRT6), plays an essential role in the regulation of oxidative stress, mitochondrial function and inflammation in mammals. However, the specific role of SIRT6 in invertebrate immunity has not been reported. Here, we characterized for the first time, a sirtuin 6 homolog in 〈em〉Litopenaeus vannamei〈/em〉 (〈em〉Lv〈/em〉SIRT6), with full-length cDNA of 2919 bp and 1536 bp open reading frame (ORF) encoding a putative protein of 511 amino acids, which contains a typical SIR2 domain. Sequence and phylogenetic analysis revealed that 〈em〉Lv〈/em〉SIRT6 shares a close evolutionary relationship with SIRT6 from invertebrates. Real-time quantitative PCR analysis of 〈em〉Lv〈/em〉SIRT6 transcripts revealed that they were ubiquitously expressed in shrimp and induced in hepatopancreas and hemocytes upon challenge with 〈em〉Vibrio parahaemolyticus〈/em〉, 〈em〉Streptococcus iniae〈/em〉, lipopolysaccharide (LPS), and white spot syndrome virus (WSSV), suggesting the involvement of 〈em〉Lv〈/em〉SIRT6 in shrimp immune response. Moreover, knockdown of 〈em〉Lv〈/em〉SIRT6 decreased mitochondrial membrane potential and increased total ROS level in hemocytes, especially upon 〈em〉V. parahaemolyticus〈/em〉 challenge. Depletion of 〈em〉Lv〈/em〉SIRT6 also increased hemocytes apoptosis in terms of decreased expression of pro-survival 〈em〉Lv〈/em〉Bcl-2, but increased expression of pro-apoptotic 〈em〉Lv〈/em〉Bax and 〈em〉Lv〈/em〉Cytochrome C, coupled with high 〈em〉Lv〈/em〉Caspase3/7 activity. Shrimp were rendered more susceptible to 〈em〉V. parahaemolyticus〈/em〉 infection upon 〈em〉Lv〈/em〉SIRT6 knockdown. Taken together, our present data suggest that 〈em〉Lv〈/em〉SIRT6 plays an important role in shrimp immune response by modulating hemocytes ROS production and apoptosis during pathogen challenge.〈/p〉〈/div〉 〈/div〉