ALBERT

Description: 〈p〉Publication date: Available online 22 January 2020〈/p〉 〈p〉〈b〉Source:〈/b〉 Analytica Chimica Acta〈/p〉 〈p〉Author(s): Li-Qing Peng, Xiao-Ting Zhen, Xin Dong, Juan Yang, Yan Chen, Shu-Ling Wang, Tian Xie, Jun Cao〈/p〉 〈div xml:lang="en"〉 〈h5〉Abstract〈/h5〉 〈div〉〈p〉An on-line cyclodextrin assisted sweeping-micellar electrokinetic chromatography (CD assisted sweeping-MEKC) was developed for the simultaneous separation and concentration of four neutral analytes (erianin, dendrophenol, naringenin and scoparone) in 〈em〉Dendrobium officinale〈/em〉 Kimura et Migo (〈em〉D. officinale〈/em〉). The 〈em〉D. officinale〈/em〉 was directly determined by this on-line stacking method after simple extraction and dilution. The optimized background solution (BGS) was 50 mM phosphoric acid (PA) containing 100 mM SDS and 30% (v/v) methanol. The best separation and concentration performance of analytes dissolved in 90 mM CD and 100 mM PA was achieved in a short analysis time when injected at 50 mbar for 100 s. Compared with conventional sweeping-MEKC and MEKC method, significant improvement in enrichment efficiency was achieved by using this proposed method. A series of validation studies of the present method was performed under the optimal conditions. Good linearities were obtained with the correlation coefficients in the range of 0.994-0.999, the detection limits were ranged from 13-40 ng/mL. Sensitivity enhancement factors (SEFs) were in the range of 28.5-46.8 compared with traditional injection (injection time 3 s). Therefore, the proposed method was successfully applied for the separation and concentration of neutral analytes in real samples.〈/p〉〈/div〉 〈/div〉 〈h5〉Graphical abstract〈/h5〉 〈div〉〈p〉〈figure〉〈img src="https://ars.els-cdn.com/content/image/1-s2.0-S000326702030088X-fx1.jpg" width="469" alt="Image 1" title="Image 1"〉〈/figure〉〈/p〉〈/div〉