Publication Date:
2020
Description:
〈p〉Publication date: March 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 98〈/p〉
〈p〉Author(s): Xifeng Qiao, Pingchao Li, Jianan He, Zeshu Yu, Jiaxing Chen, Liangge He, Xue Yu, Haoran Lin, Danqi Lu, Yong Zhang〈/p〉
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〈h5〉Abstract〈/h5〉
〈div〉〈p〉Scavenger receptors play a central role in defending against infectious diseases in mammals. However, the function of SRECII remains unknown in teleost fish. In this study, type F scavenger receptor expressed by endothelial cells-II (SRECII) cDNA sequence was first identified from 〈em〉Epinephelus coioides〈/em〉, named 〈em〉Ec〈/em〉SRECII, which contained an N-terminal signal peptide, eight EGF/EGF-like cysteine-rich motifs and a C-terminal low-complexity region. The gene location maps revealed that 〈em〉Ec〈/em〉SRECII has the conservation of synteny among selected species. Subcellular localization showed that 〈em〉Ec〈/em〉SRECII was mainly located in the cytoplasm in HEK293T cells and GS cells. In healthy 〈em〉E. coioides〈/em〉, 〈em〉Ec〈/em〉SRECII mRNA was highly expressed in spleen, skin, gill, thymus and head kidney. The relative 〈em〉Ec〈/em〉SRECII mRNA expression after 〈em〉Vibrio parahaemolyticus〈/em〉 infection was significantly up-regulated at 12 h in spleen, head kidney and thymus, but downregulated at 1 d in skin and reduced at 3 d and 1 w in spleen. Furthermore, overexpression of 〈em〉Ec〈/em〉SRECII activated NF-κB and IFN-β signaling pathway 〈em〉in vitro〈/em〉. Taken together, these results indicated that 〈em〉Ec〈/em〉SRECII could be as the potential pathogen recognition receptor for involving in bacterial infection by regulating innate immunity responses in 〈em〉E. coioides〈/em〉.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
