Publication Date:
2020
Description:
〈p〉Publication date: Available online 3 January 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology〈/p〉
〈p〉Author(s): Yuebo Zhao, Kaini Zheng, Fei Zhu〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Troponin C (TnC) is one member of the EF-hand superfamily. In many species, this gene had been identified and related functions had been elucidated. The TnC gene was still blank in the 〈em〉Scylla paramamosain〈/em〉. We obtained the TnC gene for the first time in the 〈em〉S. paramamosain〈/em〉. And we systematically analyzed the possible role of this gene in the innate immunity of 〈em〉S. paramamosain〈/em〉 while infected with white spot syndrome virus (WSSV) or 〈em〉Vibrio alginolyticus〈/em〉. The full-length 1427 bp sequence of TnC contains a 453 bp open reading frame (ORF) for encoding a 151 amino acid protein. Detection of tissue specificity of gene expression showed that the TnC was primarily expressed in muscle tissue. The expression of TnC was successfully inhibited by RNA interference technology, and several immune genes were affected. The activity of phenoloxidase and superoxide dismutase increased, and the total hemocytes counts increased after RNAi of TnC. It was found that after infection with 〈em〉V. alginolyticus〈/em〉 and WSSV, the expression of TnC in hemocytes decreased. Infected with 〈em〉V. alginolyticus〈/em〉 and WSSV, the cumulative mortality and apoptotic rate of hemocytes increased after silencing the TnC gene. Our results indicate that TnC takes participate in the innate immunity of 〈em〉S. paramamosain〈/em〉 and may plays a different role in the antiviral and antibacterial immune response.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
