Publication Date:
2019
Description:
〈p〉Publication date: November 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 94〈/p〉
〈p〉Author(s): Yunpeng Cao, Na Jin, Mingli Fan, Caiyun Lv, Xiaojun Song, Ping Jin, Fei Ma〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Transforming growth factor-β activated kinase-1 (TAK1) is an important member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, which plays an important role in animal innate immune response. However, the 〈em〉TAK1〈/em〉 gene has yet not been reported in amphioxus to date. Here, we have identified and characterized a 〈em〉TAK1〈/em〉 gene from amphioxus (〈em〉Branchiostoma belcheri〈/em〉) (named as 〈em〉AmphiTAK1〈/em〉) with the full-length cDNA of 3479 bp, including an ORF sequence of 1905 bp, a 5′ UTR of 394 bp and a 3′ UTR of 1180 bp. Moreover, the predicted AmphiTAK1 protein contains STKc_TAK1 domain, TAB1 and TAB2/3 binding domain which are conserved among chordate, and phylogenetic analysis also shows that the AmphiTAK1 is located at the bottom of the chordate, revealing 〈em〉AmphiTAK1〈/em〉 as a new member of the 〈em〉TAK1〈/em〉 gene family. The further qRT-PCR analysis has shown that 〈em〉AmphiTAK1〈/em〉 is widely expressed in six investigated tissues (gonad, gill, hepatic cecum, intestine, muscle and notochord) of 〈em〉Branchiostoma belcheri〈/em〉, with high expression in notochord and gonad, moderate in gill and hepatic cecum. Notably, the expression level of 〈em〉AmphiTAK1〈/em〉 is significantly up-regulated after LPS stimulation. Specially, we also find that AmphiTAK1 protein can interact with AmphiTAB1 by immunoprecipitation assay. These findings reveal that AmphiTAK1 might interact with AmphiTAB1 to involve in innate immune response of 〈em〉Branchiostoma belcheri〈/em〉. Taken together, our present works provide a new insight into evolution and innate immune response mechanism of 〈em〉AmphiTAK1〈/em〉 gene in 〈em〉Branchiostoma belcheri〈/em〉.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
