Publication Date:
2019
Description:
〈p〉Publication date: October 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 93〈/p〉
〈p〉Author(s): Ying Wu, Yongcan Zhou, Zhenjie Cao, Yun Sun, Yang Chen, Yajing Xiang, Lu Wang, Shengnan Zhang, Weiliang Guo〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Interleukins (ILs) are a subgroup of cytokines, which are molecules involved in the intercellular regulation of the immune system. These cytokines have been extensively studied in mammalian models, but systematic analyses of fish are limited. In the current study, 3 IL genes from golden pompano (〈em〉Trachinotus ovatus〈/em〉) were characterized. The IL-1β protein contains IL-1 family signature motif, and four long helices (αA - αD) in IL-11 and IL-34, which were well conserved. All 3 ILs clustered phylogenetically with their respective IL relatives in mammalian and other teleost species. Under normal physiological conditions, the expression of IL-1β, IL-11, and IL-34 were detected at varied levels in the 11 tissues examined. Most of the 3 ILs examined were highly expressed in liver, spleen, kidney, gill, or skin. Following pathogenic bacterial, viral, or parasitic challenge, IL-1β, IL-11, and IL-34 exhibited distinctly different expression profiles in a time-, tissue-, and pathogen-dependent manner. In general, IL-1β was expressed at higher levels following challenge with all pathogens examined than was observed for IL-11 and IL-34. Furthermore, 〈em〉Streptococcus agalactiae〈/em〉 and 〈em〉Cryptocaryon irritans〈/em〉 caused higher levels of IL-1β and IL-11 expression than 〈em〉Vibrio harveyi〈/em〉 and viral nervous necrosis virus (VNNV). The increased expression of IL-34 caused by VNNV and 〈em〉C. irritans〈/em〉 were higher than that caused by 〈em〉V. harveyi〈/em〉 and 〈em〉S. agalactiae〈/em〉. These results suggest that these 3 ILs in 〈em〉T. ovatus〈/em〉 may play different effect pathogen type specific responses.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
