Publication Date:
2019
Description:
〈h3〉Abstract〈/h3〉
〈p〉Ansamitocins are extraordinarily potent antitumor agents. Ansamitocin P-3 (AP-3), which is produced by 〈em〉Actinosynnema pretiosum〈/em〉, has been developed as a cytotoxic drug for breast cancer. Despite its importance, AP-3 is of limited applicability because of the low production yield. 〈em〉A. pretiosum〈/em〉 strain X47 was developed from 〈em〉A. pretiosum〈/em〉 ATCC 31565 by mutation breeding and shows a relatively high AP-3 yield. Here, we analyzed the 〈em〉A. pretiosum〈/em〉 X47 genome, which is ~8.13 Mb in length with 6693 coding sequences, 58 tRNA genes, and 15 rRNA genes. The DNA sequence of the ansamitocin biosynthetic gene cluster is highly similar to that of the corresponding cluster in 〈em〉A. pretiosum〈/em〉 ATCC 31565, with 99.9% identity. However, RT-qPCR analysis showed that the expression levels of ansamitocin biosynthetic genes were significantly increased in X47 compared with the levels in the wild-type strain, consistent with the higher yield of AP-3 in X47. The annotated complete genome sequence of this strain will facilitate understanding the molecular mechanisms of ansamitocin biosynthesis and regulation in 〈em〉A. pretiosum〈/em〉 and help further genetic engineering studies to enhance the production of AP-3.〈/p〉
Print ISSN:
0343-8651
Electronic ISSN:
1432-0991
Topics:
Biology
,
Medicine
