Publication Date:
2019
Description:
〈p〉Publication date: Available online 28 March 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology〈/p〉
〈p〉Author(s): Ping Wang, Zhanying Zhang, Zhongtian Xu, Baoying Guo, Zhi Liao, Pengzhi Qi〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Toll-like receptors (TLRs) are a category of most well recognized pattern recognition molecules that act on an vital role in both innate and adaptive immunity. In the present study, a novel toll-like receptor (〈em〉Mc〈/em〉TLRw) was identified and characterized in thick shell mussel 〈em〉Mytilus coruscus. Mc〈/em〉TLRw possesses one intracellular Toll/interleukin-1 (IL-1) receptor (TIR) domain, one transmembrane region (TM), one leucine rich repeat N-terminal domain (LRR_NT) and a few of leucine-rich repeats (LRRs), which all are common in typical TLRs. 〈em〉Mc〈/em〉TLRw transcripts were constitutively expressed in all examined tissues with higher expression levels in immune related tissues, and were significantly induced in haemocytes with the challenges of live 〈em〉Vibrio alginolyticus〈/em〉, lipopolysaccharide (LPS), peptidoglycans (PGN) and β-glucan (GLU), but not induced by polyinosinic-polycytidylic acid (poly I:C). rMcTLRw exhibited affinity to LPS, PGN and GLU while no affinity to poly I:C. Further, the downstream of TLR signaling pathway myeloid differentiation factor 88a (MyD88a), interleukin-1 receptor-associated kinase-4 (IRAK4) and tumor necrosis factor receptor-associated factor 6 (TRAF6) were significantly repressed in 〈em〉Mc〈/em〉TLRw silenced mussels while challenged with LPS. These results collectively indicated that 〈em〉Mc〈/em〉TLRw is one member of TLR family and involved in immune response to against invaders by taking participate in TLR mediated signaling pathway.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
