Publication Date:
2018
Description:
〈p〉Publication date: March 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 86〈/p〉
〈p〉Author(s): Yuxiong Lai, Min Liang, La Hu, Zicheng Zeng, Hai Lin, Gao Yi, Ming Li, Zhaoyu Liu〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉RIG-I-like receptors (RLRs) play a key role in antiviral and inflammatory responses. Increasing evidence indicates that ubiquitination is crucial for regulation of RIG-I signaling pathway. Several ubiquitin ligases were reported to be involved in RIG-I-mediated signal transduction. In the present study, we demonstrated zebrafish RING finger protein 135 (zbRNF135) was a critical player in the regulation of RIG-I signaling pathway. 〈em〉zbRNF1〈/em〉35 mRNA was widely expressed in different tissues of zebrafish. The expression of 〈em〉zbRNF135〈/em〉 was up-regulated post poly(I:C) treatment 〈em〉in vivo〈/em〉 and in 〈em〉vitro〈/em〉. Furthermore, the expression profiles of RIG-I signaling pathway related genes (〈em〉LGP2〈/em〉, 〈em〉MDA5〈/em〉, 〈em〉RIG-I〈/em〉, 〈em〉MAVS〈/em〉, 〈em〉TRAF3〈/em〉, 〈em〉IRF3〈/em〉 and 〈em〉IRF7〈/em〉), together with its downstream molecules (〈em〉IFN1〈/em〉, 〈em〉ISG15〈/em〉, 〈em〉Mx〈/em〉 and 〈em〉PKR〈/em〉), were up-regulated by overexpression of zbRNF135 in ZF4 cells. Luciferase and ubiquitination assays revealed that overexpression of zbRNF135 facilitated zebrafish RIG-I (zbRIG-I)-mediated IFN1 promoter activation by mediating K63-linked ubiquitination of zbRIG-I. The co-immunoprecipitation assay showed that zbRNF135 specifically interacted with zbRIG-I. Our study indicated that zbRNF135 participated in innate immune response through modulating RIG-I signaling pathway.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
