Publication Date:
2018
Description:
〈p〉Publication date: March 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 86〈/p〉
〈p〉Author(s): Fanxing Meng, Yuanyuan Zhang, Jianbo Zhou, Ming Li, Ge Shi, Rixin Wang〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Unlike the normal anadromous lifestyle, Chinese native Dolly Varden char (〈em〉Salvelinus malma〈/em〉) is locked in land and lives in fresh water lifetime. To explore the effect of freshwater adaption on its immune system, we constructed a pooled cDNA library of hepatopancreas and spleen of Chinese freshwater Dolly Varden char (〈em〉S. malma〈/em〉). A total of 27,829 unigenes were generated from 31,233 high-quality transcripts and 17,670 complete open reading frames (ORF) were identified. Totally 25,809 unigenes were successfully annotated and it classified more native than adaptive immunity-associated genes, and more genes involved in toll-like receptor signal pathway than those in complement and coagulation cascades (51 vs 3), implying the relative more important role of toll-like receptors than the complement system under bacterial injection for the freshwater Dolly Varden char. These huge different numbers of TLR and complement system identified in freshwater Dolly Varden char probably caused by distinct evolution pressure patterns between fish TLR and complement system, representative by 〈em〉TLR3〈/em〉 and 〈em〉TLR5〈/em〉 as well as 〈em〉C4〈/em〉 and 〈em〉C6〈/em〉, respectively, which were under purifying and positively selecting pressure, respectively. Further seawater adaptation experiment and the comparison study with our library will no doubt be helpful to elucidate the effect of freshwater adaption of Chinese native Dolly Varden char on its immune system.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
