Publication Date:
2018
Description:
〈p〉Publication date: January 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Soil Biology and Biochemistry, Volume 128〈/p〉
〈p〉Author(s): Charles R. Warren〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Phospholipids are isolated from crude lipid extracts by silica solid phase extraction (SPE), but for soils we don't know if phospholipids are the only fatty acid-based lipids present in the polar lipid fraction. Lipids extracted from three soils were fractionated with a silica SPE protocol commonly used for soils, with “neutrals” eluted by chloroform, “glycolipids” eluted by acetone, and “phospholipids” eluted by methanol. Fatty acid-based lipids were identified and quantified by liquid chromatography-mass spectrometry. Phospholipids were recovered in the methanol fraction, but this fraction also included betaine lipids. In two soils the methanol fraction was 3–6% betaine lipid while in one soil betaine lipids accounted for 48% of lipids in the methanol fraction. Clearly the fraction obtained by eluting lipids from silica with methanol is not purely phospholipid but can contain significant amounts of other polar lipids.〈/p〉〈/div〉
〈/div〉
Print ISSN:
0038-0717
Electronic ISSN:
1879-3428
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
