ALBERT

Description: 〈p〉Publication date: October 2018〈/p〉 〈p〉〈b〉Source:〈/b〉 Cytokine, Volume 110〈/p〉 〈p〉Author(s): Mehdi Tourani, Maryam Habibzadeh, Ahmad Karkhah, Javad Shokri-Shirvani, Ladan Barari, Hamid Reza Nouri〈/p〉 〈div xml:lang="en"〉 〈h5〉Abstract〈/h5〉 〈div〉〈p〉Peptic ulcer is a lesion in the mucosa of the digestive tract affecting many people all around the world. Recent investigations have indicated that produced inflammatory cytokines such as TNF-α and IL-1β in response to gastric infection by 〈em〉Helicobacter pylori〈/em〉 play an important role in the development of peptic ulcer. With regard to the significance of these cytokines in peptic ulcer development and the high prevalence of this disease in the developing countries, this study aimed to investigate the association of TNF-α and IL-1β with peptic ulcer in the presence of 〈em〉H. pylori〈/em〉. This case-control study enrolled 61 patients with peptic ulcer disease (PUD) as cases and 59 people without peptic ulcer (NPUD) as controls. Blood samples and endoscopic biopsies were collected. 〈em〉H. pylori〈/em〉 infection was confirmed by using rapid urease test (RUT), specific IgG measurement and histopathological examination. Then, IL-1β and TNF-α levels were evaluated using enzyme linked immunosorbent assay (ELISA). The seropositivity of 〈em〉H. pylori〈/em〉 was 62.5% in the studied population, while by considering RUT and histopathological examination along with specific-IgG antibody, 〈em〉H. pylori〈/em〉 infection decreased to 56.7%. In addition, 〈em〉H. pylori〈/em〉 infection was significantly (OR = 0.37; 95% CI = 0.17–0.82; P = .02) associated with peptic ulcer development. The TNF-α level in PUD and infected 〈em〉H. pylori〈/em〉 subjects was significantly higher than that of control and un-infected 〈em〉H. pylori〈/em〉 individuals. However, no significant difference of IL1β level was observed between PUD and control groups as well as between 〈em〉H. pylori〈/em〉 infected and un-infected individuals. Interestingly, IL-1β level in PUD patients without 〈em〉H. pylori〈/em〉 infection was significantly higher than infected ones. Moreover, a significant correlation between specific-IgG antibody with TNF-α level was observed. Taken together, our results showed that increased level of TNF-α could probably play pivotal role in pathogenesis of peptic ulcer in the presence of 〈em〉H. pylori〈/em〉 infection. These findings also highlighted the importance of IL-1β in the absence of 〈em〉H. pylori〈/em〉 infection in peptic ulcer development.〈/p〉〈/div〉 〈/div〉