Publication Date:
2012-04-16
Description:
16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose. Content Type Journal Article Pages 1-6 DOI 10.1007/s12223-012-0135-4 Authors Jaime A. Rosero, Institute of Animal Physiology and Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic Lenka Štrosová, Institute of Animal Physiology and Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic Jakub Mrázek, Institute of Animal Physiology and Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic Kateřina Fliegerová, Institute of Animal Physiology and Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic Jan Kopečný, Institute of Animal Physiology and Genetics, Vídeňská 1083, 142 20 Prague 4, Czech Republic Journal Folia Microbiologica Online ISSN 1874-9356 Print ISSN 0015-5632
Print ISSN:
0015-5632
Electronic ISSN:
1874-9356
Topics:
Biology
