ALBERT

Description:    Lipase-catalyzed acidolysis of a modified pine nut oil (MPNO)—the pine nut oil was obtained from Pinus koraiensis Siebold & Zucch.—with capric acid was studied in a continuous packed bed reactor (PBR) using Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. The MPNO containing pinolenic acid (PLA) at the sn -2 position of the triacylglycerol (TAG) backbone was prepared by lipase-catalyzed redistribution of pine nut oil using Novozym 435 from Candida antarctica . The effects of the water content in the reaction mixture and the molar ratio of substrates on the extent of the acidolysis reaction as a function of residence time in a PBR were investigated. The water content of the reaction mixture significantly influenced both the rate of acidolysis and the degree of acyl migration, but the molar ratio of substrates affected only the rate of acidolysis. The optimum water content and molar ratio for synthesis of the structured lipid containing PLA at the sn -2 position and capric acid at the sn -1,3 positions of the TAG backbone were 0.04%, and 1:5 (MPNO to capric acid), respectively. Content Type Journal Article Category Original Paper Pages 1-6 DOI 10.1007/s11746-012-2040-z Authors Jong-Hun Choi, Department of Food and Nutrition, Korea University, Jeongneung-dong, Seongbuk-Gu, Seoul 136-703, Republic of Korea Byung Hee Kim, Department of Food Science and Technology, Chung-Ang University, Anseong, 456-756 Republic of Korea Seung In Hong, Department of Food and Nutrition, Korea University, Jeongneung-dong, Seongbuk-Gu, Seoul 136-703, Republic of Korea Yangha Kim, Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 120-749 Republic of Korea In-Hwan Kim, Department of Food and Nutrition, Korea University, Jeongneung-dong, Seongbuk-Gu, Seoul 136-703, Republic of Korea Journal Journal of the American Oil Chemists' Society Online ISSN 1558-9331 Print ISSN 0003-021X