Publication Date:
2023-01-13
Description:
Data contain source data for Figure 5c from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. Retrovirally transduced CFU-E cells were incubated with increasing Epo concentrations for 14 h and proliferation was measured by [3H]-thymidine incorporation.
Keywords:
Detector raw counts; Event label; Experiment_ppERK_Treatment_a; Experiment_ppERK_Treatment_b; Experiment_ppERK_Treatment_c; Experiment_ppERK_Treatment_d; Thymidine incorporation; Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 1; Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 2; Treatment: Amount concentration, protein kinase B; Treatment: chemical concentration (biological activity), of Erythropoietin
Type:
Dataset
Format:
text/tab-separated-values, 800 data points
