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Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity (1992)

Wolf, D. A. ; Goodwin, T. J. ; Schwarz, R. P.

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Publication Date: 2019-07-13

Description: High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.

Keywords: Aerospace Medicine

Type: Journal of tissue culture methods : Tissue Culture Association manual of cell, tissue, and organ culture procedures (ISSN 0271-8057); 14; 2; 51-7

Format: text

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