ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hit 1 - 1 | 1 hit

Select All Export

Electronic Resource

Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase (2004)

White, E.Lucile ; Ross, Larry J ; Cunningham, Amanda ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 232 (2004), S. 0

add to mindlist on the mindlist

Details

ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The gene for dihydrofolate reductase of Mycobacterium tuberculosis was amplified by polymerase chain reaction (PCR) from M. tuberculosis H37Rv strain genomic DNA. The protein was expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter. Active enzyme was obtained by refolding from guanidine HCl and after a single chromatography step the sample was 〉99% homogeneous with a specific activity of ∼15.5 μmol min−1 mg−1. Mass spectrometry analysis confirmed the expected mass of 17.6 kDa. Gel filtration of the enzyme indicated that it was a monomer. Steady-state kinetic parameters were determined and the effect of pH and KCl on the enzyme examined. Methotrexate and trimethoprim inhibited the enzyme.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/S0378-1097(04)00038-2

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

hit 1 - 1 | 1 hit